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Hitesh Dave

Congratulations Nik...and Hard luck to Tiffany...tough competition..everyone really need well deserved weekend...Enjoy guys and once again thanks to all to make this event a big success..:-)

Favourite Thing: Hello Junior Scientists, I always think science is everywhere and all around us. We just need to look the things closely and always ask questions how and why this happened?? You will definitely find scientist in your soul. I work with pathology department where we see effects of new drugs(future medicines) on tissues and body fluids. I just love to prepare the glass slides of tissues in the lab and compare any effects our new drug got on body tissues in toxicology studies.

My CV

Education:

(2011- till date): M.Sc.-Applied Toxicology,Uni. of Surrey, UK, 1999- 2000: Post Graduation (Masters of Science) Gujarat Uni., India. M.Sc.-Zoology with specialization in Reproductive Physiology & Endocrinology. Studied Cell biology, Fisheries, Biomedical technology and biological sciences subjects 1996-1998: Graduation (Bachelors of Science) Gujarat University, India. BSc. Zoology (First Class) Studied Chemistry, Physics and Biology..

Qualifications:

B.Sc.(Zoology), M.Sc. (Zoology), DISM, cBiol, MSB, Pursuing M.Sc. (Applied Toxicology) UK

Work History:

May ‘00- Sept. ‘06: Torrent Research Centre, INDIA, Scientist-II Work Profile: Handled various responsibilities in the fields of Preclinical Safety Evaluation area.I was involved in Management and scheduling of Routine pathological investigations including euthanasia, necropsy, collection of tissues, trimming, embedding and sectioning of the tissue blocks, staining, quality check of the tissues & slides, Clinical Chemistry, Haematology, preparation of laboratory solutions, stains etc..

Current Job:

Nov.2006 to Present: GlaxoSmithKline Research Centre, Senior Scientist, Pathology and Toxicology, Safety Assessment dept. UK Work Profile: Actively involved as Study Histologist in routine histological processing for toxicology studies. Participating in routine necropsies, tissue selecting and trimming, processing, embedding and microtomy tasks. Performing specialise histology techniques i.e. immunohistochemistry, image analysis, method development work etc..

Employer:

GlaxoSmithKline Pharmaceutical Company (GSK R&D) , Ware, UK

Me and my work

I work in Pathology division of safety assessment department. It sounds complicated..rgt??..okay let’s make it simple…Safety assessment means to assess the safety of our newly discovered and designed drugs/molecules/compounds which will be going to become medicines for patients. It’s so simple..we can’t test new chemical straight to humans..so we need to check the safety of those chemicals on non-human species….and if the chemical is promising and pass all the safety tests..it will go to clinic to check safety and efficacy (Will it cure the illness??) on patients…

Safety Assessment department has two main divisions. 1). Toxicology:- Study of toxic (poisonous) new chemicals and test them on non-human species or models like small laboratory animals to evaluate safety and make decisions about appropriate dose of drug when it goes to patients. (e.g. If your doctor will tell you to take medicines three times a day..you might think  why can’t I take all once a day???The answer is NO…as our body is designed to accomodate only certain amout of dose at certain period..so every action has a reason in science. 2). Pathology:- In Pathology we have two units.1). Histotechnology:- Histo means tissues..study of tissues…2). clinical pathology: Study on biological fluids like blood, urine, faeces etc…  During or After toxicology department finishes their studies on non-human species Pathology department’s role starts. We analyse the body fluids like blood and urine to check and assess the effects of drug on body…and we collect and prepare the body tissues for microscopic evaluation to check any changes in cells or tissue structure due to drug…

Regulatory Environment:-And most important is we work with regulated and strict good laboratory practice environment where we follow all proper guidelines and most of us has home office UK liscences to perform certain laboratory procedures….It means we ensure the drug or molecule pass the safety and goes to patient will have higher integrity and confidence…Our thinking is every person should do more, feel better and live longer…

My Typical Day

My day to day routine in Histotechnology lab starts when toxicology study completed. 1). Fixation:-We required to fix the tissues in fixatives to prevent cells from autolysis. 2). Selection:-Once tissues been fixed we select the appropriate tissue for our sample( Because we can’t put whole kidney or liver on small glass slide!!)..3). Processing:- Our selected tissue is still soft and we need to provide hard medium to it so we can easily take small micron sections. so we pass them under series of chemicals. 4). Embedding:- We embed the tissues in liquid wax and keep the tissue block on Ice plate to make it solid . 5). Sectioning:- To see and check tissue and its cells under microscope we prepare small 3-4 microns sections on glass slides.5). Staining:- In order to differentiate cell nucleus and cytoplasm we stain them and now its ready for microscopy..really easy isn’t it??….now you all are masters…

Study Histologist: – As part of Study Histologist role i am direct point of contact for the Study Director, Study Pathologist and the designated Tox Support responsible licensee. The intention is to provide continuity of awareness of all study specific requirements from necropsy through to archiving. I am responsible for keeping the Study Director and Pathologist informed of continuous scheduling  and histology process with the study e.g. slide completion dates, special stain results,  missing organs, as well as presence of e.g. any significant macroscopic observations at necropsy. Being Study Histologist i attend pre study briefings to familiarise myself and group with the study design and purpose. The role will also involve the checking of the draft protocol and possible attendance at protocol review sessions. At a practical level, i will be responsible for ensuring the transfer of specimens to supporting divisions i.e. DMPK,IPT,Clin Path, as well as for the prompt filing of wet tissue and blocks after the study has been completed in addition to collating and quality check of slides to ensure we deliver best quality tissue slides to pathologists as per study protocol requirements.

 Involved in routine maintenance of lab instruments, Internal Audit rota for all areas of division, acting as safety rep for formaldehyde and xylene monitoring. Working to create and update photographic guides for microtomy which will help to judge quality of slides. Fully trained for working on human biological samples.

Study Supervisor: Successfully completed training for study supervisor role (Next to study director) in Toxicology department. Started working as study director for Pathology based investigative studies.

Image Analysis: Working with imaging division for operation of Scanscope Slide Scanning Instrument, Spectrum database and Imagescope software as part of Digital pathology system.

What I'd do with the money

I still do lots of student tours at our laboratory where students have hands-on experience with real science world.

I would like to arrange workshops and lab experience visits for school students starts with the younger age..because I believe they are future and I wants them to feel the real beauty of science…

My Interview

How would you describe yourself in 3 words?

Scientist, Folk Dancer, Cricketer

Who is your favourite singer or band?

I am a fan of Indian bollywood music…A.R. Rehman..(Jai ho…Jai ho…)

What is the most fun thing you've done?

Rock Climbing Mountaineering at Kerala, India.

If you had 3 wishes for yourself what would they be? - be honest!

1). I would like to have new Latest BMW. 2). I wants to open folk dance institute. 3). I definitely wants to go back to school…

What did you want to be after you left school?

Army Officer

Were you ever in trouble in at school?

Generally I was obedient student; but I remembered I bunk the school class only once when i was 16 yrs old and went for cinema with my class-mates…and I found my dad’s friend (Strict and scary uncle) at ticket window. I can’t say more…My dad was there in school second day with my class teacher and principle..because I was monitor of the class and we were total 12-15 out of 40 students….now you guys are clever to understand what would have happend???

What's the best thing you've done as a scientist?

I simplified the routine procedure of skin tissue preparation and make it better to evaluate much better area of skin tissue….

Tell us a joke.

Boy: My Father’s Name is LAUGHING and my Mother’s name is SMILING…… Teacher: You must be KIDDING………. Boy: Nope, That’s my brother………I am JOKING….

Other stuff

Work photos:

This image shows tissue, wax block and slide

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Tissue Processor for processing tissues under chemicals and wax

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Tissue Embedder

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Tissue Block embedded in wax

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Microtome to take thin 3-4 microns sections

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Slide stainer to stain the slides with various stains

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Stained slides

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Slide under light microscope with different stains

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Electron Microscope

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Immunohistochemistry staining shows antigen antibody binding

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